Composite

Part:BBa_K3712018:Design

Designed by: Yifan Li   Group: iGEM21_HUST2-China   (2021-10-20)


Optimized BLP-7-27 ELP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to understand the function of the optimized BLP-7 and 27ELP in the connection of the two, so we chose to connect the two with the flexible peptide GGGGS.


Source

We synthesize this gene fragment at GenScript.

References